Transformation of corynespora cassiicola by agrobacterium tumefaciens. Corynespora cassiicola and stemphylium lycopersici. A simple and efficient method to prepare 11chlorocyclopropyl21,2,4triazol1ylethan1one via nucleophilic substitution of 2chloro11chlorocyclopropylethanone and 1,2,4triazole is described. Frontiers compounds released by the biocontrol yeast. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. Cookies are small text files that contain a string of characters and uniquely identifies a browser. It is not yet known whether the same strain of this pathogen attacks both the crops. The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Corynespora cassiicola corynespora cassiicola berk. Phaeohyphomycosis caused by a plant pathogen, corynespora. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola.
Distribution and molecular characterization of corynespora. Species in the genus induce corynespora leaf spot in bleeding hearts clerodendrum thomsoniae. The fungus causing target spot disease, corynespora cassiicola berk. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was. Wei is a cosmopolitan plant pathogen, especially abundant in the tropics. Few studies referred to the formation of chlamydospores by c. Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. Wei from infected rubber leaves in sungai buloh two isolates and serdang two isolates were cultured on potato dextrose agar pda. First report of corynespora cassiicolaincited target spot on cotton in alabama. Conner, alabama cooperative extension system, auburn university, al 36849. Zhang, department of entomology and plant pathology, auburn university, al 36849.
Corynespora leaf blight of cotton in brazil and its management. Since that time, corynespora cassiicola has been identified as the cause of leaf spots on weeping fig ficus benjamina 3. Sensitivity loss by corynespora cassiicola, isolated from. University of florida, ifas central florida research and education center apopka cfrecapopka research report, rh9312.
A new foliar disease, corynespora leaf spot, has been. Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. To the best of our knowledge, this study represents the first report of the sensitivity of previously unexposed greenhouse collected isolates of c. Chase the first report of a corynespora leaf spot of a foliage plant was made in 1973 on zebra plant aphelandra squarrosa 4. Frontiers genomewide analysis of corynespora cassiicola. One of the major fungal plant diseases in the tropical and subtropical regions of the world is corynespora cassiicola. Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. Inter simple sequence repeat issr and rdnaits sequence markers along. It attacks several plant species including soybean. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. In recent years, corynespora leaf blight is on the increase in cotton and has become an important disease in the state of mato grosso, brazil. Pathogenicity of corynespora cassiicola on different hosts in amazonas state. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot.
The necrotrophic fungus corynespora cassiicola berk. Differentiation of corynespora cassiicola and cercospora. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. The objective of this study was to test the formation of chlamydospores by. On cotton, it begins a small brickred spot which then leads to the formation of large 14 to 1in. On the inoculated stem there are sunken, pale greyish, elliptical lesions, measuring 1. Department of plant pathology, bidhan chandra krishi viswavidyalaya. Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Genomewide analysis of corynespora cassiicola leaf fall. Only a single, historical clinical case of human infection caused by c. Their structures including absolute configurations were determined by. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. Morphological features and inter simple sequence repeat issr polymorphism were employed to analyse 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in malaysia.
It is a member of the mitosporic ascomycota, a heterogeneous group of ascomycotic fungi whose common characteristic is the absence of a sexual state. Sobers the first report of corynespora cassiicola berk. Abstract the fungus corynespora cassiicola, causal agent of target spot in soybeans, can. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. Naphthalenones and depsidones from a spongederived. In this study, iprodione ec 50 values ranged from 0. Corynespora cassiicola causing leaf spots on coleus barbatus corynespora cassiicola causing leaf spots on coleus barbatus fernandes, r. Two pathogens were isolated from tomatoes with severe chlorosis and necrosis in northwestern argentina. Wei type species of the genus 100 described species found on diverse substrates. Structural analysis of cassiicolin, a hostselective. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer. General information about corynespora cassiicola coryca.
Pdf symptomatology, hosts, and pathogenicity of corynespora. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. Synthesis and characterization of 11chlorocyclopropyl. Conventional pcr for detection of corynespora cassiicola. Chemical control of the soybean target spot corynespora. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Diversity of corynespora cassiicola isolates and changes. This pathogen is common on a wide range of host plants 1,2,6,7,9,and is considered a predominantly tropical pathogen despite its common occurrence as a leaf, stem, and. Symptomatology, hosts, and pathogenicity of corynespora cassiicola sintomatologia, hospedeiros e patogenicidade.
Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. Download pdf abstractexcerpt full text pdf contributor notes. The purpose of this research is to find the reliable means to determine the risk posed to agriculture in the pacific basins by the. They are sent to a computer by website operators or third parties. All four isolates exhibited variability in colony colour and growth rates on artificial media pda, psa, rlea and wa. The organism was isolated and identified as corynespora cassiicola according to its. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. Keywords corynespora, leaf spot, chilli capsicum annuum l. Cultural and reproductive variability of corynespora. A total of 618 isolates of corynespora leaf spot fungus corynespora cassiicola collected from 24 commercial cucumber greenhouses in 12 cities in ibaraki prefecture, japan, were tested for their sensitivity to boscalid.
Corynespora leaf spot or target spot is caused the fungus corynespora cassiicola, which causes leaf spots on cucumber, sweet potato, soybean, tomato and cotton. A study was conducted to analyse the diversity among c. It is widely used as a medicine against liver and digestion ailments in brazil. Genomic insight into pathogenicity of dematiaceous fungus. First report of corynespora cassiicolaincited target spot. It has been reported in more than 70 host plants including economically important crops such as rubber tree, tomato, cucumber, cotton, soybean, tobacco, cocoa, cowpea,1. However, the underlying factors that contribute to the. Development of a lamp method for detecting the n75s mutant. Sensitivity of corynespora cassiicola from soybean to. Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4. Wei was greatly enhanced when the cultures were first scraped after 3 days growth and then grown for an additional 3 days in continuous light.
Soybean target leaf spot, caused by the fungus corynespora cassiicola, is controlled especially by leaf application of fungicides. Pdf sensitivity of corynespora cassiicola from soybean. Development of a lamp method for detecting the n75s mutant in sdhiresistant corynespora cassiicola. He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. Diagnostic features of corynespora cassiicola and its. Corynespora cassiicola on chilli in west bengal, india. Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis.
Detection of corynespora cassiicola in hevea rubber tree. The objectives of the present investigation were to verify the genotypic and phenotypic variation between the. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. Corynespora cassiicola is a species of fungus well known as a plant pathogen. Corynespora cassiicola, stemphylium lycopersici, chlorosis, fungal diseases of plants, necrosis, plant pathogenic fungi, tomatoes, argentina abstract.
Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and. Pdf pathogenicity of corynespora cassiicola on different hosts in. The title compound is the key intermediate required for the synthesis of prothioconazole, a promising agricultural fungicide. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. Transformation of corynespora cassiicola by agrobacterium. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. Corynespora cassiicola causing leaf spots on coleus. Corynespora leaf fall, caused by corynespora cassiicola, is one of the most important diseases in rubber hevea brasiliensis plantations. Author links open overlay panel jiamei zhu a b 1 lingyan zhang a b 1 hong li a b yangyang gao a b wei mu a b feng. Morphological and inter simple sequence repeat issr. Two new naphthalenones, corynenones a and b 1 and 2, and one new depsidone, corynesidone e 3, together with one known depsidone, corynesidone a 4 and two known diphenyl ethers, corynethers a 5 and b 6, were isolated from the spongederived fungus corynespora cassiicola xs20090i7. Most browsers are initially set up to accept cookies, since this is required by most website owners in order to access their sites. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has.
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